The next monoclonal antibodies had been bought away from Pharmingen (Hillcrest, Calif
The next monoclonal antibodies had been bought away from Pharmingen (Hillcrest, Calif Phone staining and you can move cytometry. Single-cell suspensions were prepared from different lymphoid organs and incubated for 10 min at 10 6 cells/20 ?l on ice in staining buffer (phosphate-buffered saline [PBS] containing 0.5% bovine serum albumin [BSA] and 0.01% NaN3) with …
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